{"id":2440,"date":"2025-01-06T14:59:55","date_gmt":"2025-01-06T20:59:55","guid":{"rendered":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/?page_id=2440"},"modified":"2025-01-06T15:01:01","modified_gmt":"2025-01-06T21:01:01","slug":"colloquium-ben-donovan","status":"publish","type":"page","link":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/colloquium-ben-donovan\/","title":{"rendered":"Colloquium &#8211; Ben Donovan"},"content":{"rendered":"<h3>Ben Donovan, Center for Cancer Research, National Cancer Institute<\/h3>\n<h4>Real-Time Visualization of Spliceosome Assembly Reveals Basic Principles of Splice Site Selection<\/h4>\n<p><strong>Summary<\/strong><\/p>\n<ol>\n<li>We developed a kinetic model of splice site selection based on single-molecule imaging of the U2AF heterodimer in vitro and in vivo.<\/li>\n<li>The RNA helicase DDX42 accelerates U2AF dissociation while in complex with the spliceosome assembly at high-affinity U2AF binding sites.<\/li>\n<li>Overall, this work establishes how the spliceosome achieves high specificity to bona fide splice sites while still allowing for efficient forward progression.<\/li>\n<\/ol>\n<p><strong><br \/>\nJoint Colloquium with Dept. of Biological Sciences<br \/>\nThursday, January 16, 2025<br \/>\n<\/strong>Talk: 4:00 PM, MRB III 1220<br \/>\nLight snacks provided at 3:45 PM in the lobby<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ben Donovan, Center for Cancer Research, National Cancer Institute Real-Time Visualization of Spliceosome Assembly Reveals Basic Principles of Splice Site Selection Summary We developed a kinetic model of splice site selection based on single-molecule imaging of the U2AF heterodimer in vitro and in vivo. The RNA helicase DDX42 accelerates U2AF dissociation while in complex with&#8230;<\/p>\n","protected":false},"author":185,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"spay_email":"","_links_to":"","_links_to_target":""},"tags":[8],"acf":[],"_links":{"self":[{"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/pages\/2440"}],"collection":[{"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/users\/185"}],"replies":[{"embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/comments?post=2440"}],"version-history":[{"count":2,"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/pages\/2440\/revisions"}],"predecessor-version":[{"id":2442,"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/pages\/2440\/revisions\/2442"}],"wp:attachment":[{"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/media?parent=2440"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/physics-astronomy\/wp-json\/wp\/v2\/tags?post=2440"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}