{"id":2892,"date":"2024-03-26T00:11:37","date_gmt":"2024-03-26T00:11:37","guid":{"rendered":"https:\/\/as.vanderbilt.edu\/biological-sciences\/?p=2892"},"modified":"2024-03-29T14:30:59","modified_gmt":"2024-03-29T14:30:59","slug":"illuminating-a-critical-step-in-initiating-dna-replication-in-eukaryotes","status":"publish","type":"post","link":"https:\/\/as.vanderbilt.edu\/biological-sciences\/illuminating-a-critical-step-in-initiating-dna-replication-in-eukaryotes\/","title":{"rendered":"Illuminating a critical step in initiating DNA replication in eukaryotes"},"content":{"rendered":"<p>The research teams of <b>Brandt Eichman<\/b>, Professor and Chair of Biological Sciences and Professor of Biochemistry, and\u00a0<b>Walter Chazin<\/b>, Professor of Biochemistry, worked together to understand how DNA replication is initiated in eukaryotes. Using Vanderbilt\u2019s state-of-the-art instrumentation in the <a href=\"https:\/\/www.vanderbilt.edu\/csb\/facilities\/cryo-electron-microscopy\/\" target=\"_blank\" rel=\"noopener noreferrer\">Center for Structural Biology\u2019s Cryo-Electron Microscopy Facility<\/a>, Eichman, Chazin, and their colleagues provided detailed visualizations of DNA polymerase \u03b1\u2013primase, the multifunctional protein responsible for synthesis of RNA-DNA primers needed for genome duplication humans, in action.<\/p>\n<figure id=\"attachment_2894\" aria-describedby=\"caption-attachment-2894\" style=\"width: 805px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" class=\"wp-image-2894\" src=\"https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-300x79.png\" alt=\"\" width=\"805\" height=\"212\" srcset=\"https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-300x79.png 300w, https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-1024x269.png 1024w, https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-768x202.png 768w, https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-1536x403.png 1536w, https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26000413\/swivel-2048x538.png 2048w\" sizes=\"(max-width: 805px) 100vw, 805px\" \/><figcaption id=\"caption-attachment-2894\" class=\"wp-caption-text\">Cryo-electron microscopy images of DNA polymerase \u03b1\u2013primase at various stages of DNA synthesis revealed a remarkable range of motion between subunits<\/figcaption><\/figure>\n<p>Eichman and Chazin&#8217;s reflections on this research can be found <a href=\"https:\/\/medschool.vanderbilt.edu\/basic-sciences\/2024\/03\/15\/qa-illuminating-a-critical-step-in-initiating-dna-replication-in-eukaryotes\/\">here<\/a>.<\/p>\n<p>The paper \u201c<a href=\"https:\/\/www.nature.com\/articles\/s41594-024-01227-4\" target=\"_blank\" rel=\"noopener noreferrer\">A mechanistic model of primer synthesis from catalytic structures of DNA polymerase \u03b1\u2013primase<\/a>\u201d was published in\u00a0<i>Nature Structural &amp; Molecular Biology<\/i>\u00a0on March 15, 2024.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The research teams of Brandt Eichman, Professor and Chair of Biological Sciences and Professor of Biochemistry, and\u00a0Walter Chazin, Professor of Biochemistry, worked together to understand how DNA replication is initiated in eukaryotes. Using Vanderbilt\u2019s state-of-the-art instrumentation in the Center for Structural Biology\u2019s Cryo-Electron Microscopy Facility, Eichman, Chazin, and their colleagues provided detailed visualizations of DNA&#8230;<\/p>\n","protected":false},"author":27,"featured_media":2901,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[8,4],"acf":[],"jetpack_featured_media_url":"https:\/\/cdn.vanderbilt.edu\/vu-cas\/wp-content\/uploads\/sites\/5\/2024\/03\/26001923\/DSA.png","_links":{"self":[{"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/posts\/2892"}],"collection":[{"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/users\/27"}],"replies":[{"embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/comments?post=2892"}],"version-history":[{"count":7,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/posts\/2892\/revisions"}],"predecessor-version":[{"id":2903,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/posts\/2892\/revisions\/2903"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/media\/2901"}],"wp:attachment":[{"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/media?parent=2892"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/categories?post=2892"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/as.vanderbilt.edu\/biological-sciences\/wp-json\/wp\/v2\/tags?post=2892"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}